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Fig. 2. fat-3 deleted mutants do not synthesize fat-3 mRNA. (A) Schematic of the fat-3(lg8101) and fat-3(qa1811) deletions and the primers used for fat-3 mRNA analysis. (B,C) No wild-type fat-3 mRNA is detected in fat-3(lg8101) or fat-3(lg8101)/fat-3(qa1811) mutant animals. (B) RT-PCR of total mRNA from fat-3(lg8101) or wild-type animals with primers a and b, in the presence (+) or in the absence (-) of reverse transcriptase. Predicted PCR products: cDNA, 281 bp; genomic DNA, 326 bp. (C) RT-PCR with primers b and c of total mRNA from fat-3(lg8101), wild-type or fat-3(lg8101)/fat-3(qa1811) animals. Predicted PCR products: cDNA, 999 bp; genomic DNA, 1,451 bp.





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