JCS00823 Movies

Files in this Data Supplement:

• Movie 1 (9.3mb)
• Movie 2 (9.2mb)
• Movie 3 (3.6mb) -

  Movies 1-3. The dynamics of vimentin intermediate filaments and b3 integrin-rich focal contacts in TrHBMEC transfectants (see Fig. 2A-C for still images). TrHBMECs co-expressing YFP-b3 integrin and CFP-vimentin were followed over 60, 55 and 60 minutes respectively in the live state. The YFP signal is shown in red while the CFP signal is shown in green. Images were collected every 5 minutes. The display rate is 6 frames/second.

• Movie 4 (8.5mb) -

  Movie 4. The dynamics of vimentin intermediate filaments and b3 integrin-rich focal contacts following initiation of flow and during the subsequent 120 minutes (see Fig. 3 for still images). TrHBMECs co-expressing YFP-b3 integrin and CFP-vimentin were followed over 120 minutes in the live state following the exposure to flowing medium at 12 dynes/cm². The YFP signal is shown in red and the CFP signal is shown in green. Frames were collected every 10 minutes. The display rate is 6 frames/second.

• Movie 5 (8.5mb) -

  Movie 5. The dynamics of vimentin filament and b3 integrin-rich focal contacts subject to flow for over 3 hours (see Fig. 5 for still images). TrHBMECs co-expressing YFP-b3 integrin and CFP-vimentin was subjected to flowing medium at 12 dynes/cm² and then viewed live for the period 120-220 minutes after the initiation of shear stress. The YFP signal is shown in red while the CFP signal is shown in green. Frames were collected every 10 minutes. The display rate is 6 frames/second.