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Fig. 2. The dynamics of vimentin IFs and ß3 integrin-rich FCs in TrHBMEC transfectants. TrHBMECs co-expressing YFP-ß3 integrin and CFP-vimentin were followed over periods up to 60 minutes in the live state. A-C reflect different types of interactions and relative behaviors of vimentin IFs and FCs in double transfectants. (A,B) The signals for the three time points were coded blue, green and red, with the final panel reflecting all three images merged. In A, a YFP-ß3 integrin-containing FC moves centripetally in concert with vimentin IF (arrow). (B) Vimentin IFs elongate from the perinuclear zone towards an FC (arrow) at the edge of the cell. (C) A newly assembled FC (arrow) appears and establishes contact with vimentin IF. Scale bar: (A) 5 µm, (B,C) 3 µm. A, B and C can be viewed in video form (movies 1, 2 and 3, http://jcs.biologists.org/supplemental/).





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