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Fig. 3. The dynamics of vimentin IF and ß3 integrin-rich FCs following initiation of flow and during the subsequent 105 minutes. (A) TrHBMEC co-expressing YFP-ß3 integrin and CFP-vimentin were followed over a period of 105 minutes in the live state after the exposure to flowing medium at 12 dynes/cm2. The YFP images at 0, 45 and 105 minutes were coded in blue, green and red, respectively, and then overlaid. Note that FCs are dynamic during the viewing period as revealed by the rainbow color effect in D. Scale bar: 5 µm. This can be viewed in video form (movie 4, http://jcs.biologists.org/supplemental/).





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