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Fig. 2. SDS-PAGE and immunoblot detection of the very large (>600 kDa) protein desmoyokin. On SDS-PAGE-separation (5.5% gel) of total proteins, desmoyokin (arrows) is detected in cultured kidney epithelial cells of line MDCK (lanes 1 and 2) as a positive control and in bovine lens fiber cells (lanes 3 and 4). Molecular weights of reference proteins examined in parallel are indicated on the left.





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