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Fig. 3. Identification of the N-cadherin-associated protein complexes in lens fiber plasma membranes, demonstrated by immunoprecipitation, SDS-PAGE (8%) and immunoblotting. (Lane 1) Coomassie-Blue-stained major polypeptides of the immunoprecipitate obtained from water-insoluble, 1%-Triton X-100-soluble fractions from Sepharose-bound antibodies against N-cadherin. (Lanes 2-7) Proteins of immunoprecipitates subjected to SDS-PAGE were probed by immunoblotting with antibodies against N-cadherin (lane 2; N-cad), cadherin-11 (lane 3; Cad-11), {alpha}-catenin (lane 4; {alpha}-cat), ß-catenin (lane 5; ß-cat), plakoglobin (lane 6; PG) and p120ctn (lane 7; p120). Antibodies against E-cadherin showed no immunoreactivity; similarly controls using Sepharose-bound uncoated or coated with antibodies against desmoplakins or desmocollins were negative. Molecular weight markers are indicated on the left.





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