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Fig. 2. Nontransgenic left and right primary ventricular cultures after transient transfection. (A,B) Phase contrast image of X-gal-stained rat primary cultures prepared from right (A) and left (B) free ventricular walls after transfection with the Mlc3f-nlacZ-2 construct. Similar numbers of ß-galactosidase positive nuclei are observed in left and right cultures. (C,D) Low magnification bright-field view of right (C) and left (D) rat primary cultures after transfection with the Mlc3f-nlacZ-2 construct and X-gal staining. Scale bars: 25 µm.





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