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Fig. 4. Mlc3f-nlacZ transgenes are unmethylated in all compartments of the heart. (A) Ethidium bromide-stained gel showing restriction endonuclease digested Mlc3f-nlacZ-2E DNA isolated from different regions of the heart or fast skeletal muscle with HpaII (H) or MspI (M) endonucleases. (B) Southern blot hybridisation of the gel in A using a 2 kb lacZ probe, showing that the reporter gene is unmethylated to an equivalent degree in all samples. EDL, extensor digitorum longus (fast skeletal muscle); LA, left atrium; LV, left ventricle; OFT, right ventricular outlet; RA, right atrium; RV, right ventricle.





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