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Fig. 5. Trichostatin A treatment does not modify Mlc3f-nlacZ-2 transgene expression status. Co-immunohistochemistry of primary ventricular cardiomyocyte cultures following treatment with trichostatin A and sodium butyrate for 3 days, showing Hoechst staining to identify all nuclei per field, anti-ß-galactosidase (red), anti-acetylated histone H4 (green) and the overlay of anti-ß-galactosidase and anti-acetylated histone H4 (merged). Note that although the number of nuclei testing positive for acetylated histone H4 increases after trichostatin A treatment, no change in transgene expression status is observed (see also Table 1). Scale bar: 25 µm.





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