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Fig. 1. mGluR5 displays anchorage through its C-terminus. Typical trajectories (red lines) on a hippocampal neuron growth cone (A) and on fibroblast lamellipodium (B,C) of latex beads coupled to mGluR5-myc (A,B) or a C-terminal deletion mutant (C) superimposed on a DIC image of the cell. Trajectories in A and B present anchorage as an irreversible transition between Brownian diffusion and linear retrograde movement. The point of the trajectory corresponding to the transition is depicted by a yellow cross. Recording time, 125-200 seconds; sampling time, 25 images/second. (D-F) Plots of diffusion versus time corresponding to the trajectories in (A-C). For each timepoint, the diffusion coefficient was computed from the slope of the MSD calculated for 40 points. Note the sudden drop in diffusion values in D and E, depicted by arrows and corresponding to the yellow crosses in A and B. (G-I) Plots of the anchorage coefficient versus time corresponding to the trajectories in (A-C). For each timepoint, the anchorage coefficient was computed as the ratio between the mean diffusion during the 20 seconds before and after that timepoint. The maximum of this coefficient (black arrows) thus corresponds to the drop in diffusion values depicted by the corresponding arrows in D and E, whereas the coefficient remains low during all the recording time for mGluR5-DelC (F). (J,K) Plots of the MSD versus time for the control trajectory recorded on fibroblast depicted in B, computed before and after anchorage. Note the linearity of the MSD before anchorage, and the positive curvature displayed after.
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