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Fig. 6. Effect of 480-Cbl expression on PDGF-mediated activation of Src. Wild-type,
c-Cbl- and 480-Cbl-expressing NIH 3T3 cells were lysed following serum
starvation or after 1 and 5 minutes of treatment with PDGF. Aliquots of the
lysates were directly subjected to SDS-PAGE and probed by western blotting
with anti-phospho-Shc (Y239) and anti-Shc (A) or anti-phospho-Stat3 (Y704) and
anti-Stat3 (B) as indicated.
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