Fig. 2. p75^NTR is expressed in the developing inner ear. (A) p75^NTR mRNA expression was detected by whole mount in situ hybridization in HH18 chicken embryos. Arrowheads point to major patches of p75 expression, within the otic epithelium and in the CVG (cvg). The microphotograph shows a 60 µm parasagittal vibratome section. At least 12 embryos were sampled in three different experiments. (B) Immunolocalization of p75^NTR protein was positive in the otic epithelium and in the CVG (arrowheads). The microphotograph shows a 25 µm parasagittal section from an HH18 chicken embryo. At least 10 embryos were sampled in three different experiments. (C,D) Detection of apoptotic cells by TUNEL labelling in a 25 µm parasagittal cryostat section from an HH18 embryo. D shows a magnification of the CVG area. (E) 2.5 µm optic section of an otic vesicle cultured in the presence of 4 nM NGF. Apoptotic positive cells were visualised by TUNEL labelling. C and E arrowheads point to apoptotic cells in the otic epithelium and CVG. C and E microphotographs are representative of at least three different experiments performed in triplicate. A, anterior; D, dorsal; cvg marks the CVG area. Bar, 100 µm (A, B, C and E), 50 µm (D).

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