Fig. 5. Remodeling of TMRM fluorescence changes in a virtual cell. (A) Remodeling of TMRM fluorescence in a STS-treated cell in the absence of caspase inhibitor z-VAD-fmk. TMRM fluorescence changes were remodeled with a _M depolarization of 32 mV and a _P depolarization of 10 mV as detected with Dibac₄(3). The calculated average intensity of TMRM is plotted in black (scale on the left). The modeling of _M and _P kinetics is plotted in dark and light gray, respectively (scale on the right). Experimentally determined values from cells treated with 3 µM STS are shown for comparison (open squares). (B) Remodeling of a cell treated with 3 µM STS and 200 µM z-VAD-fmk. TMRM fluorescence changes were remodeled with a _M depolarization of 32 mV in the absence of _P depolarization. (C) Remodeling of TMRM fluorescence changes in cells treated with 3 µM STS and 5 µM oligomycin. TMRM fluorescence changes were remodeled with a _M depolarization of 65 mV and a _P depolarization of 10 mV as detected with Dibac₄(3) (data not shown). Necrosis was remodeled to occur 83 minutes after the release of cyt-C and was simulated with a total depolarization of both potentials at that time. (D) Experimentally determined values for the steady-state level of TMRM fluorescence intensity after cyt-C-GFP release. The values were calculated using the sigmoidal Boltzmann equation as fit function for the single-cell kinetics of TMRM fluorescence from the experiments presented in Figs 1, 2 and 3. (^*P<0.05 compared to STS-treated cells).

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