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Fig. 3. Patches and caps of Fc ${gamma}$ RII colocalize with CD55 but not with TfR. Distribution of Fc ${gamma}$ RII (A, red) and CD55 (B, green) during Fc ${gamma}$ RII crosslinking. (C) The merged image of A and B shows colocalization of CD55 and Fc ${gamma}$ RII in receptor patches (yellow). (D) The merged image of Fc ${gamma}$ RII (red) and CD55 (green) reveals the colocalization of the proteins in Fc ${gamma}$ RII cap (yellow). Distribution of Fc ${gamma}$ RII (F, red) and TfR (G, green) during Fc ${gamma}$ RII crosslinking. (H) Merged images of Fc ${gamma}$ RII (red) and TfR (green) show the separation of the proteins during Fc ${gamma}$ RII patching. E shows representative line profiles of intensity of fluorescent labeling of crosslinked Fc ${gamma}$ RII and CD55 (upper plot) and crosslinked Fc ${gamma}$ RII and TfR (lower plot). Seven such profiles were generated for each analyzed cell to estimate the protein colocalization. Bar, 5 µm.

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