Fig. 6. The cleavage of NuMA, lamin A/C, lamin B, PARP-1 and procaspase-8, -3 and -7 is inhibited in the presence of z-DEVD-FMK, z-VEID-FMK or z-IETD-FMK. HeLa cells grown on a monolayer were treated with Fas antibody and PD 98059 for 24 hours in the presence or absence of 100, 10, 1 or 0.1 µM z-DEVD-FMK, z-VEID-FMK or z-IETD-FMK. Cells were prepared for SDS-PAGE and immunoblotted with NuMA, lamin A/C, lamin B, PARP-1, caspase-8, caspase-3 or caspase-7 antibody. Note, that z-DEVD-FMK, z-VEID-FMK and z-IETD-FMK inhibit the cleavage of NuMA, lamins, PARP-1 and pro-caspase-8, -3 and -7 identically and dose dependently.

Return to article