Fig. 4. Effect of inhibitors of the temperature-signaling cascade in A. polypoides on temperature- and ABA-induced short-term stimulation of O₂ consumption and filtration rate. O₂ consumption and dye filtration were recorded for 2 hours before (control) and after exposure of A. polypoides fragments to heat stress, ABA or 8-Br-cAMP. EGTA-AM (15 µM) and 8-Br-cADPR (10 µM) were added 30 minutes before ABA (20 µM). Bupivacaine (BUPI; 1 mM) and GdCl₃ (50 µM) were added during heat stress (24°C for 30 minutes). The calculated slope of the linear regression curve (R0.98) is compared with that of the respective control, i.e. the same fragment prior to stress induction or addition of chemicals. Data are the mean±s.d. of five experiments.

Return to article