Fig. 4. Inhibition of GSK-3 during mitosis causes spindle defects and chromosome mis-alignment. HeLa cells were incubated with 10 µM or 30 µM of the GSK-3 inhibitors SB-216763 and SB-415286, respectively for 90 minutes. DNA, blue; tubulin, green. (A) Untreated metaphase cell; (B) SB-216763-treated metaphase cell; (C,D) SB-415286-treated metaphase cell; (E) Mitotic cell treated with 40 mM LiCl for 90 minutes; (F) Mitotic cell treated for 90 minutes with 1 nM taxol. Bar, 10 µm. (G) Effect of treating HeLa cells with SB-415286 for 16 hours. Bar, 10 µm.

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