Fig. 3. C6-conditioned media increases NFB expression in BBMEC monolayers. BBMEC monolayers were incubated for three days in C6-CM or RA-CM and then subjected to 24 hours of normoxia or hypoxic stress (1% O₂). NFB was immunoprecipitated and detected by western blotting. C6-CM significantly increases NFB expression under normoxic conditions; RA-CM also significantly increases NFB expression compared with MEM/F12. After 24 hours of hypoxia, NFB levels increase in both MEM/F12 and RA-CM-treated samples. There is no significant change in NFB expression in hypoxic C6-CM-treated samples compared to normoxic samples. ^#P<0.05 and ^##P<0.01 versus normoxic MEM/F12, ^*P<0.05 and ^***P<0.001 versus comparable normoxic treatment, n=3.

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