Fig. 1. Effect of krh1 krh2 mutations on FLO11 induction, invasive growth and cell morphology. (A) RNA was isolated from wild-type strain SKY763 (lane 1), krh1 strain SKY.k1-2B (lane 2), krh2 strain SKY.k2-4C (lane 3), krh1 krh2 strain HS182-1D (lane 4), gpa2 strain BS13B (lane 5) and krh1 krh2 gpa2 strain HS183-3D (lane 6). The RNA was transferred to nylon membrane, hybridized with a FLO11 probe, and rehybridized with an ACT1 probe. The relative amount of FLO11 RNA, normalized to ACT1 RNA, is shown below each lane. (B) Wild-type strain SKY763 (WT), krh1 strain SKY.k1-2B (krh1), krh2 strain SKY.k2-4C (krh2), krh1 krh2 strain HS182-1D (krh1 krh2), gpa2 strain BS13B (gpa2), krh1 krh2 gpa2 strain HS183-3D (krh1 krh2 gpa2), flo11 strain HS184-5A (flo11), and krh1 krh2 flo11 strain HS197-13D (krh1 krh2 flo11) were patched onto YEPD/2.5% agar medium, incubated for 4 days at 25°C, and photographed before (Total growth) and after (Invasive growth) rubbing the surface of the plate with a glass rod under a stream of water. (C) Wild-type strain BS1B (KRH1 KRH2) and double mutant strain HS154-3D (krh1 krh2) were diluted 1:20 from an overnight saturated culture into fresh YEPD medium and incubated at 30°C with shaking for 2 days. (D) Wild-type strain BS1B (KRH1 KRH2) and double mutant strain HS154-3D (krh1 krh2) were patched onto YEPD plates and incubated at 30°C for 5 days. Magnification: upper panel, 1x; lower panel, 25x.

Return to article