Fig. 2. Effect of krh1 krh2 mutations on heat shock sensitivity and sporulation. (A) Wild-type strain W3031B (open bar), krh1 strain G102-5C (filled bar), krh2 strain G103-4A (shaded bar), and krh1 krh2 strain H147-3C (hatched bar) were grown to saturation for 2 days in synthetic complete medium, incubated at 50°C for 20 minutes, and diluted and plated to determine the percentage survival. Values shown are the mean and standard deviation from three independent experiments. (B) The following strains were incubated in sporulation medium for 3 days and the percentage sporulation was determined by visual inspection: krh1 krh2 diploid strain H174 containing plasmids YEp181-FLKRH1 and YEp112-KRH2 (open bar, n=2); strain H174 containing plasmids YEplac181 and YEp112-KRH2 (filled bar, n=5); strain H174 containing plasmids YEp181-FLKRH1 and YEplac112 (shaded bar, n=2); and strain H174 containing plasmids YEplac181 and YEplac112 (hatched bar, n=5). Values shown are the means from independent experiments.

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