Fig. 4. Relationship between KRH1 and KRH2 and SCH9. (A) Wild-type strain W3031B, sch9 strain B14-53B, krh1 krh2 strain H147-3C, and krh1 krh2 sch9 strain H148-6A, all transformed with vector YEplac112, were grown to saturation for 2 days in synthetic dropout medium lacking tryptophan, incubated at 50°C for 20 minutes, and diluted and plated to determine the percentage survival. Value for W3031B is represented by the open bar; value for B14-53B is represented by the filled bar; value for H147-3C is represented by the shaded bar; value for H148-6A is represented by the hatched bar. Values shown are the mean and standard deviation from three independent experiments. (B) Strains with the following genotypes were streaked out for single colonies onto YEPD medium and grown for 2 days at 30°C: H147-3C (krh1 krh2), H152-28A (gpa2 krh1 krh2 sch9) and H137-22A (gpa2 sch9).

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