Fig. 5. Relationship between KRH1 and KRH2 and TPK2. (A) RNA from wild-type strain BS1B transformed with YEplac112 (lane 1), tpk2 strain BS3B transformed with YEplac112 (lane 2), krh1 krh2 strain HS154-3D (lane 3), and krh1 krh2 tpk2 strain HS161-4C (lane 4) was isolated and hybridized as described in the legend to Fig. 1A. (B) RNA from wild-type strain BS1B (lanes 1,2) and tpk2 strain BS3B (lanes 3,4) transformed with either plasmid pG2CT-112.2 (lanes 2,4) or vector YEplac112 (lanes 1,3) was isolated and hybridized as described in the legend to Fig. 1A. (C) krh1 krh2 strain HS154-3D and krh1 krh2 tpk2 strain HS161-4C were diluted 1:20 from an overnight saturated culture into fresh YEPD medium and incubated at 30°C with shaking for 2 days. (D) RNA from wild-type strain BS1B (lanes 1,2) and krh1 krh2 strain HS154-3D (lanes 3,4) transformed with either plasmid YEpTPK2.2 (lanes 2,4) or vector YEp351 (lanes 1,3) was isolated and hybridized as described in the legend to Fig. 1A.

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