Fig. 10. Y27632 accelerates wound closure and inhibits both Golgi polarization and MTOC polarization of wound-edge NIH 3T3 cells. (A) Wounded NIH 3T3 monolayers not treated (left panel) or treated with Y27632 (right panel) were fixed and stained for actin 15 minutes post wounding. (B) As described in (A) but fixation and labeling were performed 4 hours post wounding. (C) Percentage of Golgi polarization and (D) percentage of MTOC polarization: Golgi and MTOC orientated towards the wound were determined at 3 hours post wounding for cells treated with Y27632 (short hashed bars). Golgi polarization (C) and MTOC polarization (D) of non-drug treated woundedge cells were included as a control: blue-grey bars (C) and white bars (D), respectively (see also legend on the right side of the graph; n is the number of Golgi (C) or MTOC (D) observed and the second number, after the slash, indicates the number of separate experiments). Bar, 80 µm.

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