Fig. 2. Nocodazole, cytochalasin D and taxol effect on Golgi and MTOC polarization in wound-edge NIH 3T3 cells. Percentage of Golgi (A) or MTOC (B) orientated towards the wound was determined at 3 hours post wounding in cell monolayers treated with nocodazole (blue bars), cytochalasin D (pink bars) or taxol (green bars). Cell polarity of wound-edge cells of non-drug treated monolayers was included as a control of Golgi polarization (grey bar) (A) or MTOC polarization (white bar) (B) (see also legend at the bottom of the graphs). (C) Quantification data. The first number corresponds to the number of Golgi (A) or MTOC (B) observed and the second, in parentheses, is the number of separate experiments. (D) Golgi polarization of wound-edge cells treated with nocodazole at 300 nM and 16 µM. Cells were fixed and stained for tubulin (top panels) and for the Golgi apparatus with anti-ß-COP antibody (bottom panels).

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