Fig. 3. Arp2/3 and WASP-family proteins are not involved in MTOC polarization of wound-edge NIH 3T3 cells. MTOC polarity, at the edge of the wound, of cells expressing Scar1-WCA, Scar1-2-3-deltaA and N-WASP-deltaA: MTOC reorientation to face the wound was evaluated when wound-edge NIH 3T3 cells were microinjected with the five expression vectors encoding for the WASP-family deletion constructs described earlier (Magdalena et al., 2003). (A) The microinjected cells expressing the GFP-fusion protein are shown (top panels, from left to right): Scar1-deltaA, Scar2-deltaA and Scar3-deltaA. Cells were fixed and stained for the MTOC with the anti-pericentrin antibody (bottom panels). (B) The percentage of MTOC orientated towards the wound was determined at 3 hours post wounding for cells microinjected with the expression vectors encoding for the WASP-family deletion constructs. MTOC polarization of non-microinjected wound-edge cells was included as a control (white bar) (see legend on the right side of the graph; n is the number of MTOC observed and the second number, after the slash, indicates the number of separate experiments). Bar, 40 µm.

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