Fig. 8. ST638 inhibits wound closure as well as Golgi polarization but does not prevent the MTOC polarization of wound-edge NIH 3T3 cells. (A) Wounded NIH 3T3 monolayers not treated (left panel) or treated with ST638 (right panel) were fixed and stained for actin 9 hours post wounding. (B) Percentage of Golgi polarization and (C) percentage of MTOC polarization: Golgi and MTOC orientated towards the wound were determined at 3 hours following wounding for cells treated with ST638 (pink bars). Golgi polarization (B) and MTOC polarization (C) of non-drug treated wound-edge cells were included as a control: grey bars (B) and white bars (C), respectively (see also legend on the right side of the graph; n is the number of Golgi (B) or MTOC (C) observed and the second number, after the slash, indicates the number of separate experiments). Bar, 80 µm.

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