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Fig. 9. Overexpression of IRSp53 does not prevent Golgi polarization, but inhibits lamellipodia formation and migration of the wound-edge NIH 3T3 cells. The microinjected NIH 3T3 cells expressing the myc-tagged IRSp53 are shown in the top panels of sections A, B and C. Cells were fixed at 2 hours post wounding and labeled for Golgi (lower panel) (A) or actin (lower panel) (B). (C) Cells were fixed at 4 hours post wounding and labeled for actin (middle panel), and the upper and middle panels were merged and shown at the bottom. Bar, 40 µm.





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