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Fig. 1. Production of nidogen-binding site constructs and controls. The site for interaction between laminin-1 and nidogen-1 has been localized to LE module 4 of domain III of the laminin {gamma}1 chain (Mayer et al., 1993). Two different FLAG fusion proteins (indicated with flags) were constructed and modified with the BM40 signal peptide to ensure secretion to the extracellular space, {gamma}1III3-5 coding for LE modules 3-5 and {gamma}1V1-3 comprising three LE modules of domain V.





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