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Fig. 4. Immunoblot for laminin-1 and nidogen-1 in embryoid body total lysates. A
rabbit polyclonal antibody against laminin-1 and nidogen-1 was used to detect
these proteins in total lysates prepared from 12-day-old retinoic-acid-treated
embryoid bodies in SDS. Equal loading and transfer to nitrocellulose were
confirmed by Ponceau Red staining of the membrane (not shown).
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