Fig. 7. Laminin-1 localization in F9 wild-type embryoid bodies treated with the FLAG fusion protein 1III3-5. Embryoid bodies were transferred from a spinner culture system into single wells at day 2 and then either supplied with additional FLAG fusion protein 1III3-5 at a concentration of 10 µg ml^-1 (A) or not (B). At day 8 of culture, whole-mount staining with a rabbit polyclonal antiserum against laminin-1 was performed. Representative images show either the embryoid body surface in the upper panel (two images; bar, 100 µm) or part of a section through the embryoid body periphery in the lower panel. Images from 12 different embryoid bodies from each culture condition are shown. Bar, 25 µm.

Return to article