Fig. 4. Formation of SpII*, FANCA and XPF foci in the nucleus depended upon the time after treatment of the cells with 8-MOP plus UVA light. Normal human lymphoblastoid cells were treated with 8-MOP plus UVA light (6 kJ m^-2), fixed at the indicated times post-treatment and stained independently using either anti--spectrin, anti-FANCA or anti-XPF antibodies. (A) The percentage of nuclei showing multiple SpII*, FANCA and XPF foci was counted over a period of 0-24 hours after treatment. Nuclei in 100 cells were counted at each time point. (B) The average number of damage-induced SpII*, FANCA and XPF foci per nucleus was counted at time points from 0-24 hours after treatment. In each case, 100 nuclei were counted. Vertical lines indicate ± s.e.m.

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