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Fig. 4. Colocalization of dynamin2-EGFP and dynamin1-EGFP in disappearing
dsRed-clathrin puncta. (A) dsRed-clathrin TIR-FM image. Scale bar, 5 µm.
(B) Dynamin2-EGFP TIR-FM image. (C) Five sequential images (300 milliseconds
per frame) enlarging area outlined in A. (D) Five sequential images enlarging
area outlined in B. (E) Graphic depiction of the disappearance of eight
dsRed-clathrin and dynamin2-EGFP containing spots; values are presented as the
mean ± s.e.m. of the average fluorescence per unit area relative to
maximum value obtained for each spot minus the minimum value for each. (F, G)
Five images (300 milliseconds per frame) demonstrating the behavior of
dsRed-clathrin (F) and dynamin1-EGFP (G) prior to and during internalization.
Images depicted represent frames 0, 50, 100, 150 and 200. (H) Graphic
depiction of the disappearance of eight dsRed-clathrin and dynamin1-EGFP
containing spots; values are presented as the mean ± s.e.m. of the
average fluorescence per unit area relative to maximum value obtained for each
spot minus the minimum value for each. Values depicted were taken from an
aligned data set of 
40 seconds. In E and H, the fluorescence traces of
each of the eight spots evaluated were temporally aligned to the start of
dynamin1/2-EGFP fluorescence decrease.
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