Fig. 8. Distribution of clathrin-mediated endocytosis in the basal plasma membrane of migrating cells. (A) TIR-FM image of the basal plasma membrane ECFP-Mem distribution in a migrating MDCK cell co-transfected with dsRed-clathrin and ECFP-Mem. Cell border was drawn around the entire region of fluorescent signal. (B) dsRed-clathrin TIR-FM image of the same cell overlying the cell border drawn in A. The cell is apparently migrating in the direction denoted by the arrow. (C) The relative dsRed-clathrin fluorescence intensity per unit area divided by the relative ECFP-Mem fluorescence intensity per unit area within three regions of the migrating cell. The average of six cells ± s.e.m. is plotted. (D) The quantification of the relative dsRed-clathrin spot number per unit area within the three cell regions. The average ± s.e.m. is plotted (three cells, 1507 spots). (E) The quantification of the relative dsRed-clathrin spot disappearance per unit area within the three cell regions. The average ± s.e.m. is plotted (three cells, 237 spots).

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