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Fig. 4. mRNA in situ hybridization of TN-C. No signal for TN-C mRNA can be detected in the skeletal muscle (cross-sectional section) either (A) after the immobilization or (B) after the high-intensity treadmill running of eight weeks, whereas in the tendon (longitudinal section) (C-E), mechanical loading regulates the mRNA expression of TN-C. (C) A strong signal can be detected from the normal Achilles tendon (longitudinal section), (D) the expression almost disappears after the three-week immobilization, whereas (E) a strong singal for TN-C mRNA is detectable after the high-intensity treadmill running of eight weeks. Magnification x5.





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