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Fig. 3. TES localises to the tips of actin filaments. (A) Rat-1 fibroblasts stably expressing GFP-tagged full-length TES were stained with TRITC-labelled phalloidin to visualise actin stress fibres. Solid arrows indicate focal adhesions; dotted arrows indicate areas of cell-cell contact. Insets are enlarged regions as denoted. (B) Actin stress fibres were disrupted by treating Rat-1 fibroblasts stably expressing GFP-tagged full-length TES with 2 µM (top panel) or 10 µM (lower panel) latrunculin B for 24 hours. Cells were then fixed and stained with TRITC-labelled phalloidin.





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