Fig. 4. Cell-cycle-dependent assembly and disassembly of PCM-1 granules and aggregates. (A) A6 cells were triple stained with anti-XPCM-1 pAb (red), FITC-conjugated anti--tubulin mAb (green) and DAPI (blue). Lower panels represent only PCM-1 signals. At interphase, PCM-1 granules were scattered around the cytoplasm, showing significant concentration around the centrosomes (a). When cells entered the mitotic phase, PCM-1 granules gathered around two divided centrosomes very intensely (b) and then gradually began to disappear (c,d). At the end of the mitotic phase, PCM-1 granules again gradually appeared around the centrosomes (e). Bar, 10 µm. (B) Time-lapse images of the dynamic behavior of the large aggregates of GFP fusion proteins with a truncated XPCM-1 (aa 745-1128; Fig. 2C) during mitosis. Elapsed time is indicated at the top (in minutes). The large aggregates (arrows) decreased in size when cells entered the mitotic phase with concomitant rounding-up and then recovered to the original size at the interphase. Bar, 10 µm.

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