Fig. 2. CNN is associated with centrosomal MTOCs in dd4^S spermatocytes. (A-C) Wild-type meiosis showing CNN (red) localized at the spindle poles during wild-type meiosis. Microtubules are stained green and DNA is stained blue. Cysts are shown at prometaphase (A), anaphase (B) and telophase/cytokinesis (C). (E-H) CNN localization in dd4^S meiocytes. (D) Spermatocytes in early meiosis in which discrete bodies containing CNN do not form two discrete foci but are dispersed. In this field the three uppermost cells have at least three such bodies accumulated at the centre of the asters (arrow), although some CNN-containing bodies can be dispersed (arrowhead). (E) Hemi-spindles with variable numbers of CNN-containing bodies at the focus of the asters. (F) Two cones that show constrictions around the mid-zone (arrowheads). Punctate CNN staining is found at the astral poles of both cones and hemi-spindles (arrows). Bar, 50 µm.

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