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Fig. 4. Complete lack of subunit S5a/Rpn10/p54 in {Delta}p54 mutant pupae. Total protein extracts of wild-type (lane 1) and {Delta}p54 (lane 2) pupae were fractionated on 8% SDS-PAGE and immunoblotted with a mixture of four different monoclonal antibodies specific for subunits p54, p48A, p42C and p39.





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