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Fig. 8. Accumulation of proteasomal proteins in {Delta}p54 animals. Total protein extracts prepared from a single wild-type (lane 1) or a single {Delta}p54 (lane 2) pupa (20 hours APF) were fractionated on 10% SDS-PAGE and immunoblotted with a polyclonal antibody specific for the catalytic core. The same extracts fractionated on a 8% SDS-PAGE were immunoblotted with the following polyclonal antibodies: anti-regulatory complex antibody (lanes 3 and 4), anti-glycogen phosphorylase antibody (lanes 5 and 6) and anti-karyopherin ß antibody (lanes 7 and 8).





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