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Fig. 10. hGAR22ß bridges MFs and MTs in transfected cells. COS7 cells were transiently transfected with pFLAG-hGAR22ß and triple stained with a monoclonal anti-FLAG antibody (A), a polyclonal anti-tubulin antibody (B) and phalloidin-AlexaFluor-350 (C). (D) A superimposed image, with the FLAG staining in blue, the tubulin staining in red and the actin staining in green. Triple-stained filaments, probably resulting from crosslinking by hGAR22ß, are white colored. Bar, 10 µm.





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