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Fig. 4. Localization of the CH and GAR domains in transfected cells. NIH 3T3 (A-C) or COS7 (D-I) cells were transiently transfected with pFLAG-hCHD22 (A-C), pFLAG-hGARD17 (D-F) or pFLAG-hGARD22 (G-I) and stained with a monoclonal anti-FLAG antibody (A,D,G), phalloidin-AlexaFluor-594 (B) and a polyclonal anti-tubulin antibody (E,H). (C,F,I) Superimposed images, with the FLAG staining in green and the phalloidin or tubulin staining in red. hCHD22 protein colocalized with MFs. Both hGARD17 and hGARD22 showed colocalization with MTs (C) (F,I; see insets). Bar, 15 µm.





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