Fig. 1. Expression of A in lens epithelial wholemounts by immunofluorescence. Wholemounts prepared from wild-type and A^–/– lenses were stained with a monoclonal antibody to A and an Alexa⁵⁶⁸-labeled secondary antibody (red). The DNA-binding dye TOTO-1 (green) was used to stain the epithelial cell nuclei. (A) Merged confocal micrographs of A and nuclei visualized in the wholemount of a wild-type mouse lens. (B) Lens epithelial wholemount from an A^–/– mouse. No A immunofluorescence was detected from the A^–/– lens epithelial wholemount. Bar, 25 µM.

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