Fig. 7. Confocal images of TUNEL and BrdU staining in lens epithelial wholemounts. Seven-day-old wild-type and A^–/– mice were injected with BrdU, and wholemounts were fixed 24 hours later. (A,D,G) BrdU-labeled cells. (B,E,H) TUNEL-labeled cells. (C,F,I) Merged confocal images of BrdU- and TUNEL-labeled cells in (A,D,G) and (B,E,H). In the A^–/– lens epithelial wholemounts (A,D), several BrdU-labeled nuclei, smaller in size than normal, are scattered among the pairs of normal-sized BrdU-labeled nuclei. These smaller nuclei were often seen as distinct members of a pair (arrows). The smaller nuclei were intensely stained with TUNEL reagents (B,C,E,F). Note that the BrdU-labeled nuclei having normal dimensions were not stained with the TUNEL label. In the wild-type wholemounts, smaller BrdU-labeled nuclei were not detected often (G), and the TUNEL labeling was negligible (H,I). Bars, 10 µM (A-H).

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