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Fig. 6. Western blot analysis of CDP II in different subcellular fractions.
Cell-free extract (300 g supernatant, 180 µg), nuclear (154
µg), mitochondrial (145 µg), plasma membrane (165 µg) and soluble
(185 µg) protein fractions were electrophoresed in 11% SDS-PAGE and
immunoblotted as described in Materials and Methods. Lanes: (1) cell-free
extract; (2) nuclear; (3) mitochondrial; (4) plasma membrane; (5) soluble
protein fraction.
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