|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 5. Proteolytic sensitivity of Vph1p depends on Vtc proteins. (A) 25 µg of
vacuoles from the indicated strains expressing Vph1p with chromosomally
encoded C-terminal tags were precipitated with TCA and analyzed by SDS-PAGE
and western blotting with the indicated antibodies. All strains were derived
from OMY1, that is, deficient for vacuolar proteases (
pep4,
prb1). (B) Same analysis as in A, but with whole cell extracts from
107 cells.
|