QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?

Profiling tumour angiogenesis

Interactions between tumour cells and endothelial cells play a crucial role in cancer progression and are particularly important for the generation of new blood vessels that supply tumours (tumour angiogenesis). The tumour cells are thought to produce factors that `activate' endothelial cells, promoting their proliferation and tube formation. Ralph Weichselbaum and co-workers have examined these interactions by generating expression profiles of human umbilical vein endothelial cells (HUVECs) co-cultured with U87 glioma cells (see p. 1013). They first demonstrate that the glioma cells induce an activated phenotype in the HUVECs (they proliferate and form net-like structures). The authors then use microarray analysis to reveal that the HUVECs are reprogrammed to express a variety of angiogenic receptor-ligand pairs (e.g. TGFßRII-TGFß3, FGFRII-FGF7 and CCR5-RANTES), confirming their findings with quantitative PCR and immunohistochemical staining. They also show that conditioned media from the glioma cells can activate HUVECs cultured alone and that previously activated HUVECs can activate naive HUVECs that have not been exposed to tumour cells. The tumour cells must thus activate endothelial cells by releasing soluble factors that induce autocrine growth mechanisms.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

Related articles in JCS:

Tumour-endothelium interactions in co-culture: coordinated changes of gene expression profiles and phenotypic properties of endothelial cells

Nikolai N. Khodarev, Jianqing Yu, Edwardine Labay, Thomas Darga, Charles K. Brown, Helena J. Mauceri, Reza Yassari, Nalin Gupta, and Ralph R. Weichselbaum
JCS 2003 116: 1013-1022. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?