Fig. 11. Double immunolabelling of INCENP and aurora-B kinase (A-P), or aurora-B and phosphorylated histone H3 (pH3) (Q-X), in squashed spermatocytes (INCENP, green in A-P; aurora-B kinase, red in A-P, green in Q-X; pH3, red in Q-X; DAPI, blue). (A-D) Partial projection of three focal planes throughout a diplotene spermatocyte. Chromocentres and short and faint nuclear threads (arrows) are labelled with INCENP, but not with aurora-B. (E-H) Partial projection of two focal planes throughout a late diplotene spermatocyte. Chromocentres (arrowheads) are labelled by both INCENP and aurora-B. The sex body (XY) appears faintly labelled with INCENP. (I-L) Selected metaphase I centromere. INCENP and aurora-B colocalize at a T-shaped structure. (M-P) Selected metaphase II centromere. The connecting strand traversing the centromere shows colocalization of INCENP and aurora-B. (Q-T) Late diplotene spermatocyte. Chromocentres only appear labelled with aurora-B. (U-X) Late diplotene spermatocyte. Chromocentres and the sex body (XY) are labelled with aurora-B and pH3. (A-H,Q-X) Bar in D, 10 µm. (I-P) Bar in P, 2 µm.

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