Fig. 6. Double immunolabelling of INCENP and SCP1 in prophase I squashed spermatocytes (INCENP, green; SCP1, red; DAPI, blue). (A-D) Partial projection of four focal planes throughout a zygotene spermatocyte. Short INCENP threads colocalize with short SCP1 segments representing SC portions with central element where homologous chromosomes have synapsed. (E-H) Partial projection of two focal planes throughout a pachytene spermatocyte. INCENP threads and SC central elements along autosomal bivalents colocalize. The peudoautosomal region in the sex bivalent (XY) is labelled by both proteins (arrows). (I-L) Partial projection of three focal planes throughout an early diplotene spermatocyte. INCENP threads (arrows in I) and remaining SC central elements in desynapsing autosomal bivalents (arrows in J) colocalize. Note that the INCENP threads are fainter than the corresponding SCP1 fragments. Chromocentres (arrowhead) are brightly labelled only with INCENP. (M-P) Partial projection of two focal planes throughout a late diplotene spermatocyte. Short INCENP threads (arrows in M) are very faint in comparison with the corresponding SCP1 fragments (arrows in N). Chromocentres (arrowhead) are brightly labelled with INCENP. A faint INCENP labelling of the sex body (XY) is also observed. Bar in P, 10 µm.

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