Fig. 8. INCENP expression from anaphase I up to interkinesis (INCENP, green; ACA serum, red in I; DAPI, blue). (A-C) Two focal planes throughout an early anaphase I spermatocyte. The only INCENP signals are at centromeres (arrows). (D-E) Single focal plane of a late anaphase I spermatocyte. A bright INCENP labelling is detected at the spindle midzone. Some faint and dispersed INCENP labelling is observed at centromeres. Since a short exposure time was used to accurately visualize the midzone signal, labelling at centromeres is very low. (F,G) Single focal plane of a telophase I spermatocyte. INCENP only labels the spindle midzone (arrowheads). (H-J) Partial projection of two focal planes throughout an interkinesis spermatocyte. A bright INCENP labelling is observed at chromocentres (arrows). Bar, 10 µm.

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