Fig. 9. INCENP labelling from metaphase II up to telophase II (INCENP, green; ACA serum, red; DAPI, blue). (A-C) Partial projection of five focal planes throughout a metaphase II spermatocyte. INCENP signals appear at centromeres and are concentrated in two spots (arrowheads) partially colocalizing with sister kinetochores as revealed with the ACA serum. (D-G) Selected metaphase II chromosomes. INCENP is concentrated below sister kinetochores and at an irregular connecting strand that joins them. (H,I) Early anaphase II chromosome. INCENP is concentrated at sister kinetochores (arrowheads), and some faint interrupted labelling at the connecting strand (arrow) is visible. (J,K) Partial projection of two focal planes throughout an anaphase II spermatocyte. A diffuse INCENP labelling at centromeres (arrows) is observed. (L,M) A single focal plane of a late anaphase II spermatocyte. The INCENP labelling is discerned as two parallel plates (arrowheads) at the midbody. (A-C,J-M) Bar in M, 5 µm. (D-I) Bar in I, 2 µm.

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